posted on 2018-06-04, 11:20authored byJulie J Settipani, Kal Karim, Alienor Chauvin, Si Mohamed Ibnou-Ali, Florian Paille-Barrere, A Gorban, Evgeny Mirkes, Alexander Gorban, Lee Larcombe, Michael J. Whitcombe, Todd Cowen, Sergey A. Piletsky
Molecular modelling and computational approaches were used to design (virtual) molecularly imprinted binding sited for 170 amino acids, dipeptides and tripeptides. Analysis of the binding energy of ligands to their corresponding virtual binding sites revealed a direct correlation between size of the ligand and its binding affinity. Only tripeptides were capable of forming binding sites in molecularly imprinted polymers (MIPs) that are capable, in theory, of binding the corresponding targets at micromolar concentrations. No appreciable specificity was demonstrated in binding of virtual binding sites and corresponding templates. It is possible to conclude that although tripeptide sequences are sufficiently long to form MIPs with relatively high affinity, the sequence of peptide epitopes should be substantially longer that three amino acid residues to ensure specificity of imprinted sites. This consideration will be useful for the design of highly efficient MIPs for proteins.
History
Citation
Journal of the Chinese Advanced Materials Society, 2018, pp. 1-10 (10)
Author affiliation
/Organisation/COLLEGE OF SCIENCE AND ENGINEERING/Department of Mathematics
Version
AM (Accepted Manuscript)
Published in
Journal of the Chinese Advanced Materials Society
Publisher
Taylor & Francis for Chinese Advanced Materials Society
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