University of Leicester
fcimb-09-00326.pdf (987.15 kB)

TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GInR

Download (987.15 kB)
journal contribution
posted on 2019-09-27, 09:36 authored by AS Motib, FAY Al-Bayati, I Manzoor, S Shafeeq, A Kadam, OP Kuipers, NL Hiller, PW Andrew, H Yesilkaya
Streptococcus pneumoniae is able to cause deadly diseases by infecting different tissues, each with distinct environmental and nutritional compositions. We hypothesize that the adaptive capabilities of the microbe is an important facet of pneumococcal survival in fluctuating host environments. Quorum-sensing (QS) mechanisms are pivotal for microbial host adaptation. We previously demonstrated that the TprA/PhrA QS system is required for pneumococcal utilization of galactose and mannose, neuraminidase activity, and virulence. We also showed that the system can be modulated by using linear molecularly imprinted polymers. Due to being a drugable target, we further studied the operation of this QS system in S. pneumoniae. We found that TprA controls the expression of nine different operons on galactose and mannose. Our data revealed that TprA expression is modulated by a complex regulatory network, where the master regulators CcpA and GlnR are involved in a sugar dependent manner. Mutants in the TprA/PhrA system are highly attenuated in their survival in nasopharynx and lungs after intranasal infection, and growth in blood after intravenous infection.



Frontiers in Cellular and Infection Microbiology, 2019, 9:326

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/School of Medicine/Department of Infection, Immunity and Inflammation


  • VoR (Version of Record)

Published in

Frontiers in Cellular and Infection Microbiology


Frontiers Media



Acceptance date


Copyright date


Available date


Publisher version


The Supplementary Material for this article can be found online at: The datasets generated for this study can be found in the GEO/GSE 67668.