South London Diabetes and Ethnicity Phenotyping study protocol

The primary objective  is to assess,  in  people of  black West African and white European origin with type 2 diabetes, insulin secretory  reserve  by  graded  glucose  infusion  insulin action. Secondary objectives are to assess liver and peripheral tissue insulin sensitivity for glucose metabolism; insulin sensitivity of lipolysis;  visceral,  intramyocelluar,  intrahepatocellular  and intrapancreatic  fat  accumulation;  metabolic  flexibility  during the  euglycaemic clamp using indirect  calorimetry; and  post-prandial insulin and incretin secretion during a meal tolerance test.