Opioids and anaesthetic drugs as modulators of stimulus-secretion coupling.
thesisposted on 2015-11-19, 08:44 authored by Robert. Atcheson
The cellular mechanisms by which opioid and general anaesthetic drugs produce their clinical effect remain unclear, but modulation of stimulus-secretion coupling may be important. The human neuroblastoma cell line, SH-SY5Y, is a model of a human sympathetic neuron. The uptake and release (depolarization and receptor mediated) of [3H] noradrenaline ([3H] NA) in SH-SY5Y cells has been characterized, and the role of cAMP and calcium in evoked release examined. Further studies examined the effect of opioids (including fentanyl), a volatile anaesthetic agent (halothane), and intravenous anaesthetics (thiopentone and propofol) on uptake and release of [3H] NA. SH-SY5Y cells are capable of the uptake and evoked release of [3H] NA. K+ evoked release is dependent on extracellular calcium but carbachol evoked release appears to be extracellular calcium- independent. cAMP does not have a role in the immediate evoked release of [3H] NA, but an effect on long term secretion cannot be excluded. Fentanyl inhibits the uptake and release of [3H] NA, but this effect is not opiate receptor mediated. Morphine, DAMGO, met- and leu- enkephalin had no effect on [3H] NA release, and these results indicate that opiate receptors on SH-SY5Y cells are not coupled to neurotransmitter release. Halothane inhibits K+, but not carbachol evoked [3H] NA release. In addition, halothane inhibits [3H] NA uptake but only at relatively high concentrations. Thiopentone, but not propofol, inhibits [3H] NA uptake, and both drugs inhibit [3H] NA release. The results of these studies suggest that inhibition of voltage sensitive calcium channels by general anaesthetic agents may contribute significantly towards the state of anaesthesia.
Date of award1994-01-01
Author affiliationCell Physiology and Pharmacology
Awarding institutionUniversity of Leicester