U526187.pdf (13.06 MB)
The role of the CCR5 D32 polymorphism in abdominal aortic aneurysms
thesisposted on 2014-12-15, 10:34 authored by Rebecca M. Sandford
C-C Chemokine receptor 5 (CCR5) is involved in the regulation of the inflammatory response. Abdominal aortic aneurysms (AAA) may arise as the result of a chronic inflammatory process which is influenced by genetic predisposition. The CCR5 gene is associated with a 32 base pair deletion (the Î”32 polymorphism). The aim of this study was to investigate the role of the CCR5 Î”32 polymorphism in the development of AAA.;A case control study was conducted including 285 patients with AAA and 273 control subjects. A blood sample was taken from each individual and DNA extracted. CCR5 genotype was determined using the polymerase chain reaction (PCR). Flow cytometry was used to investigate the biological activity of the Î”32 polymorphism.;There was no significant difference between the AAA and the control group in relation to the Î”32 allele frequency (AAA group10%, control group=12%, P=0.82, chi squared analysis). Genotype analysis revealed no significant difference between the groups (AAA vs controls, wild type homozygotes=82% vs 77% heterozygotes=16% vs 21%, vs Î”32 homozygotes= 2% and 2% respectively, P=33, chi squared analysis). The polymorphism was shown to be biologically active with the number of Î”32 alleles correlating with cell expression of CCR5 as detected with flow cytometry (P=<0.05).;This study demonstrates that the CCR5 Î”32 is a biologically active genetic polymorphism, however, there is no association between this polymorphism and AAA.
Date of award2008-01-01
Author affiliationCardiovascular Sciences
Awarding institutionUniversity of Leicester