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Development of competitive 'pseudo'-ELISA assay for measurement of cocaine and its metabolites using molecularly imprinted polymer nanoparticles

journal contribution
posted on 2017-08-31, 13:42 authored by Yadiris Garcia, Katarzhina Smolinska-Kempisty, Eduardo Pereira, Elena Piletska, Sergey Piletsky
The analytical test-system for cocaine, benzoylecgonine and norcocaine was developed in the ELISA format using molecularly imprinted polymeric nanoparticles (nanoMIPs) as synthetic recognition elements that were produced using a solid-phase synthesis approach. The experimental conditions of the assay were optimized using a Box-Behnken experimental design protocol. The detection of free cocaine and its metabolites was performed using a competitive binding assay in the model samples and in blood plasma. There was no cross-reactivity of the developed assay towards paracetamol and caffeine. The developed assay had a picomolar limit of detection of cocaine (LOD = 4.24 pM), which was almost three orders of magnitude lower than the LOD expected from the commercial antibody-based ELISA (3.3 nM), and other attractive features of a new assay included a long shelf-life, lower economic cost and a short production time. Therefore, it is possible to state that nanoMIPs have the potential to become the recognition elements of choice for the development of a new generation of test-systems and sensors.

Funding

The authors thank FONDECYT project No. 1160942 and Y. Garcia is thankful for the scholarship CONICYT No. 63140157 for financial support

History

Citation

Analytical Methods, 2017, 9 (31), pp. 4592-4598

Author affiliation

/Organisation/COLLEGE OF SCIENCE AND ENGINEERING/Department of Chemistry

Version

  • AM (Accepted Manuscript)

Published in

Analytical Methods

Publisher

Royal Society of Chemistry

issn

1759-9660

eissn

1759-9679

Acceptance date

2017-07-13

Copyright date

2017

Available date

2018-07-14

Publisher version

http://pubs.rsc.org/en/Content/ArticleLanding/2017/AY/C7AY01523B

Notes

The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.

Language

en

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