Development of competitive 'pseudo'-ELISA assay for measurement of cocaine and its metabolites using molecularly imprinted polymer nanoparticles
journal contributionposted on 2017-08-31, 13:42 authored by Yadiris Garcia, Katarzhina Smolinska-Kempisty, Eduardo Pereira, Elena Piletska, Sergey Piletsky
The analytical test-system for cocaine, benzoylecgonine and norcocaine was developed in the ELISA format using molecularly imprinted polymeric nanoparticles (nanoMIPs) as synthetic recognition elements that were produced using a solid-phase synthesis approach. The experimental conditions of the assay were optimized using a Box-Behnken experimental design protocol. The detection of free cocaine and its metabolites was performed using a competitive binding assay in the model samples and in blood plasma. There was no cross-reactivity of the developed assay towards paracetamol and caffeine. The developed assay had a picomolar limit of detection of cocaine (LOD = 4.24 pM), which was almost three orders of magnitude lower than the LOD expected from the commercial antibody-based ELISA (3.3 nM), and other attractive features of a new assay included a long shelf-life, lower economic cost and a short production time. Therefore, it is possible to state that nanoMIPs have the potential to become the recognition elements of choice for the development of a new generation of test-systems and sensors.
The authors thank FONDECYT project No. 1160942 and Y. Garcia is thankful for the scholarship CONICYT No. 63140157 for financial support
CitationAnalytical Methods, 2017, 9 (31), pp. 4592-4598
Author affiliation/Organisation/COLLEGE OF SCIENCE AND ENGINEERING/Department of Chemistry
- AM (Accepted Manuscript)